PrimePCR™ Probe Assay: SPAG6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

Info:   Recommended - best negative control

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058502
Assay Design:   exonic
Chromosome Location:   10:22653829-22657451question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000376624 ENST00000456231 ENST00000313311 ENST00000435326 ENST00000376603 ENST00000376601 ENST00000538630

Gene Information

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility as well as identification of proteins for targeted contraception are dependent on the identification and characterization of relevant sperm antigens. The protein expressed by this gene is recognized by anti-sperm antibodies from an infertile man. This protein localizes to the tail of permeabilized human sperm and contains eight contiguous armadillo repeats a motif known to mediate protein-protein interactions. Studies in mice suggest that this protein is involved in sperm flagellar motility and maintenance of the structural integrity of mature sperm. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2011]

Gene Symbol:   SPAG6
Gene Name:   sperm associated antigen 6
Aliases:   DKFZp434I153, MGC26276, Repro-SA-1, pf16
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000077327
Entrez:   9576
UniGene:   Hs.655170
Chromosome Mapping:   10p12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 35.910000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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