PrimePCR™ Probe Assay: RRM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058446
Assay Design:   exonic
Chromosome Location:   11:4139602-4141080question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000300738 ENST00000534285 ENST00000423050 ENST00000537197

Gene Information

This gene encodes one of two non-identical subunits that constitute ribonucleoside-diphosphate reductase an enzyme essential for the production of deoxyribonucleotides prior to DNA synthesis in S phase of dividing cells. It is one of several genes located in the imprinted gene domain of 11p15.5 an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome Wilms tumor rhabdomyosarcoma adrenocrotical carcinoma and lung ovarian and breast cancer. This gene may play a role in malignancies and disease that involve this region. [provided by RefSeq Jul 2008]

Gene Symbol:   RRM1
Gene Name:   ribonucleotide reductase M1
Aliases:   R1, RIR1, RR1
RefSeq:   NC_000011.9 NG_016277.1 NG_027992.1 NT_009237.18
Ensembl:   ENSG00000167325
Entrez:   6240
UniGene:   Hs.445705
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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