PrimePCR™ Probe Assay: PRPF19, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRPF19, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRPF19, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058424
Assay Design:   exonic
Chromosome Location:   11:60670231-60670333question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000307360 ENST00000506939 ENST00000336431 ENST00000427420 ENST00000441551 ENST00000536736 ENST00000300850 ENST00000394979 ENST00000343813 ENST00000227524 ENST00000541371 ENST00000546152 ENST00000577454

Gene Information

PSO4 is the human homolog of yeast Pso4 a gene essential for cell survival and DNA repair (Beck et al. 2008 [PubMed 18263876]).[supplied by OMIM Sep 2008]

Gene Symbol:   PRPF19
Gene Name:   PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Aliases:   NMP200, PRP19, PSO4, SNEV, UBOX4, hPSO4
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000110107
Entrez:   27339
Chromosome Mapping:   11q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.960000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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