PrimePCR™ Probe Assay: ITGAE, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058368
Assay Design:   exonic
Chromosome Location:   17:3618109-3618223question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000263087

Gene Information

Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage in the extracellular domain yielding disulfide-linked heavy and light chains. In combination with the beta 7 integrin this protein forms the E-cadherin binding integrin known as the human mucosal lymphocyte-1 antigen. This protein is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL) and in addition to a role in adhesion it may serve as an accessory molecule for IEL activation. [provided by RefSeq Jul 2008]

Gene Symbol:   ITGAE
Gene Name:   integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide)
Aliases:   CD103, HUMINAE, MGC141996
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000083457
Entrez:   3682
UniGene:   Hs.513867
Chromosome Mapping:   17p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 35.730000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download