PrimePCR™ Probe Assay: BCL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058350
Assay Design:   exonic
Chromosome Location:   18:60985331-60985468question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000398117 ENST00000333681 ENST00000589955 ENST00000444484

Gene Information

This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2 such as in the case of translocation of BCL2 to Ig heavy chain locus is thought to be the cause of follicular lymphoma. Two transcript variants produced by alternate splicing differ in their C-terminal ends. [provided by RefSeq Jul 2008]

Gene Symbol:   BCL2
Gene Name:   B-cell CLL/lymphoma 2
Aliases:   Bcl-2
RefSeq:   NC_000018.9 NG_009361.1 NT_025028.14
Ensembl:   ENSG00000171791
Entrez:   596
UniGene:   Hs.150749
Chromosome Mapping:   18q21.33,18q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.480000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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