PrimePCR™ Probe Assay: RIMS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: RIMS1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RIMS1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058298
Assay Design:   exonic
Chromosome Location:   6:73100322-73102415question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000491071 ENST00000517960 ENST00000518273 ENST00000522291 ENST00000521978 ENST00000520567 ENST00000264839 ENST00000517433 ENST00000401910 ENST00000523963 ENST00000425662 ENST00000453976 ENST00000517827 ENST00000370420 ENST00000522211 ENST00000414192 ENST00000348717 ENST00000538414

Gene Information

The protein encoded by this gene is a RAS gene superfamily member that regulates synaptic vesicle exocytosis. This gene also plays a role in the regulation of voltage-gated calcium channels during neurotransmitter and insulin release. Mutations have suggested a role cognition and have been identified as the cause of cone-rod dystrophy type 7. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq Mar 2012]

Gene Symbol:   RIMS1
Gene Name:   regulating synaptic membrane exocytosis 1
Aliases:   CORD7, KIAA0340, MGC167823, MGC176677, RAB3IP2, RIM, RIM1
RefSeq:   NC_000006.11 NT_007299.13 NG_016209.1
Ensembl:   ENSG00000079841
Entrez:   22999
Chromosome Mapping:   6q12-q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 35.870000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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