PrimePCR™ Probe Assay: SLC16A7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SLC16A7, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC16A7, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058250
Assay Design:   exonic
Chromosome Location:   12:60165049-60168441question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000552432 ENST00000547379 ENST00000552024 ENST00000548610 ENST00000261187 ENST00000548444 ENST00000543448

Gene Information

Tissues with few or no mitochondria such as erythrocytes and tumor cells depend largely on glycolysis to generate ATP. The major end products of glycolysis pyruvate and lactate must be eliminated from these cells to enable continued glycolytic flux and prevent toxic effects. H+/monocarboxylate transporters (MCTs) mediate the transport of lactate and pyruvate. Human MCT2 has a high affinity for the transport of pyruvate (summary by Lin et al. 1998 [PubMed 9786900]).[supplied by OMIM Feb 2011]

Gene Symbol:   SLC16A7
Gene Name:   solute carrier family 16, member 7 (monocarboxylic acid transporter 2)
Aliases:   MCT2
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000118596
Entrez:   9194
Chromosome Mapping:   12q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996700
y-intercept 35.500000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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