PrimePCR™ Probe Assay: COL19A1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: COL19A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: COL19A1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0058031
Assay Design:   exonic
Chromosome Location:   6:70918389-70918535question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000322773 ENST00000393344

Gene Information

This gene encodes the alpha chain of type XIX collagen a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Although the function of this collagen is not known other members of this collagen family are found in association with fibril-forming collagens such as type I and II and serve to maintain the integrity of the extracellular matrix. The transcript produced from this gene has an unusually large 3' UTR which has not been completely sequenced. [provided by RefSeq Jul 2008]

Gene Symbol:   COL19A1
Gene Name:   collagen, type XIX, alpha 1
Aliases:   COL9A1L, D6S228E
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000082293
Entrez:   1310
Chromosome Mapping:   6q12-q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.120000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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