PrimePCR™ Probe Assay: KRTAP9-1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: KRTAP9-1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KRTAP9-1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057962
Assay Design:   exonic
Chromosome Location:   17:39346648-39346745question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000398470 ENST00000377721 ENST00000334109 ENST00000391355 ENST00000391354 ENST00000571104 ENST00000570861 ENST00000576511 ENST00000573531 ENST00000568193 ENST00000564962 ENST00000565557 ENST00000565080 ENST00000561589 ENST00000561919 ENST00000567302 ENST00000565268 ENST00000409762 ENST00000409582 ENST00000429174 ENST00000413539 ENST00000258104 ENST00000409651 ENST00000394120 ENST00000409744 ENST00000409366 ENST00000410020 ENST00000410041 ENST00000381222 ENST00000381223 ENST00000329905 ENST

Gene Information

Description Not Available

Gene Symbol:   KRTAP9-1
Gene Name:   keratin associated protein 9-1
Aliases:   KAP9.1, KRTAP9L3
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000240542
Entrez:   728318
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.992800
y-intercept 37.780000
Efficiency 90

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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