PrimePCR™ Probe Assay: NOXO1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: NOXO1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NOXO1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057866
Assay Design:   exonic
Chromosome Location:   16:2031174-2031316question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000448645 ENST00000428611 ENST00000371521 ENST00000344894 ENST00000427047 ENST00000449208 ENST00000446013 ENST00000426996 ENST00000413055 ENST00000456988 ENST00000416714 ENST00000449742 ENST00000376704 ENST00000369888 ENST00000450537 ENST00000217130 ENST00000417411 ENST00000451727 ENST00000354038 ENST00000259941 ENST00000431258 ENST00000447465 ENST00000568546 ENST00000436083 ENST00000356987 ENST00000432564 ENST00000356120 ENST00000354249 ENST00000567471 ENST00000303383

Gene Information

NADPH oxidases (NOXs) catalyze the transfer of electrons from NADPH to molecular oxygen to generate reactive oxygen species (ROS). NOX organizers such as NOXO1 target NOX activators (see NOXA1; MIM 611255) to NOX and also target NOX to different subcellular compartments (Opitz et al. 2007 [PubMed 17189823]).[supplied by OMIM Mar 2008]

Gene Symbol:   NOXO1
Gene Name:   NADPH oxidase organizer 1
Aliases:   MGC20258, P41NOX, P41NOXA, P41NOXB, P41NOXC, SH3PXD5, SNX28
RefSeq:   NC_000016.9 NG_016288.1 NT_010393.16
Ensembl:   ENSG00000196408
Entrez:   124056
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.970000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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