PrimePCR™ Probe Assay: FKBP10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: FKBP10, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FKBP10, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057854
Assay Design:   exonic
Chromosome Location:   17:39976543-39976674question
Amplicon Length:   102
Splice Variants Targeted:   ENST00000321562 ENST00000455106 ENST00000544340 ENST00000566592 ENST00000568842 ENST00000567782

Gene Information

The protein encoded by this gene belongs to the FKBP-type peptidyl-prolyl cis/trans isomerase (PPIase) family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone. Alternatively spliced variants encoding different isoforms have been reported but their biological validity has not been determined.[provided by RefSeq Nov 2009]

Gene Symbol:   FKBP10
Gene Name:   FK506 binding protein 10, 65 kDa
Aliases:   FKBP65, FLJ20683, FLJ22041, FLJ23833, OI6, PPIASE, hFKBP65
RefSeq:   NC_000017.10 NG_015860.1 NT_010783.15
Ensembl:   ENSG00000141756
Entrez:   60681
Chromosome Mapping:   17q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.590000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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