PrimePCR™ Probe Assay: STXBP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057841
Assay Design:   exonic
Chromosome Location:   19:7707135-7707321question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000373887 ENST00000540010 ENST00000546084 ENST00000441779 ENST00000221283 ENST00000414284

Gene Information

This gene encodes a member of the STXBP/unc-18/SEC1 family. These genes are conserved across species from yeast to man and are believed to be involved in protein trafficking from the golgi apparatus to the plasma membrane. Mutations in this gene are associated with familial hemophagocytic lymphohistiocytosis. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq Mar 2010]

Gene Symbol:   STXBP2
Gene Name:   syntaxin binding protein 2
Aliases:   FHL5, Hunc18b, MUNC18-2, UNC18-2, UNC18B, pp10122
RefSeq:   NC_000019.9 NT_077812.2 NG_016709.1
Ensembl:   ENSG00000076944
Entrez:   6813
UniGene:   Hs.515104
Chromosome Mapping:   19p13.3-p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.790000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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