PrimePCR™ Probe Assay: GRN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GRN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GRN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057821
Assay Design:   exonic
Chromosome Location:   17:42430137-42430265question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000053867 ENST00000589265 ENST00000586443 ENST00000586242

Gene Information

Granulins are a family of secreted glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein progranulin is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active 6 kDa peptides. These smaller cleavage products are named granulin A granulin B granulin C etc. Epithelins 1 and 2 are synonymous with granulins A and B respectively. Both the peptides and intact granulin protein regulate cell growth. However different members of the granulin protein family may act as inhibitors stimulators or have dual actions on cell growth. Granulin family members are important in normal development wound healing and tumorigenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   GRN
Gene Name:   granulin
Aliases:   GEP, GP88, PCDGF, PEPI, PGRN
RefSeq:   NC_000017.10 NG_007886.1 NT_010783.15
Ensembl:   ENSG00000030582
Entrez:   2896
Chromosome Mapping:   17q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.270000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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