PrimePCR™ Probe Assay: ECE1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057778
Assay Design:   exonic
Chromosome Location:   1:21546438-21546558question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000415912 ENST00000357071 ENST00000374893 ENST00000436918 ENST00000264205

Gene Information

The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq Sep 2009]

Gene Symbol:   ECE1
Gene Name:   endothelin converting enzyme 1
Aliases:   ECE
RefSeq:   NC_000001.10 NT_004610.19 NG_013008.1
Ensembl:   ENSG00000117298
Entrez:   1889
UniGene:   Hs.195080
Chromosome Mapping:   1p36.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.990300
y-intercept 34.410000
Efficiency 108

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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