PrimePCR™ Probe Assay: GABRG3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: GABRG3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GABRG3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057749
Assay Design:   exonic
Chromosome Location:   15:27572055-27572169question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000333743 ENST00000555083 ENST00000554696

Gene Information

Gamma-aminobutyric acid (GABA) receptors are a family of proteins involved in the GABAergic neurotransmission of the mammalian central nervous system. GABRG3 is a member of the GABA-A receptor gene family of heteromeric pentameric ligand-gated ion channels through which GABA the major inhibitory neurotransmitter in the mammalian brain acts. GABA-A receptors are the site of action of a number of important pharmacologic agents including barbiturates benzodiazepines and ethanol (summary by Whiting et al. 1999 [PubMed 10414349]). For additional general information about the GABA-A receptor gene family see GABRA1 (MIM 137160).[supplied by OMIM Dec 2009]

Gene Symbol:   GABRG3
Gene Name:   gamma-aminobutyric acid (GABA) A receptor, gamma 3
Aliases:   Not Available
RefSeq:   NC_000015.9 NT_026446.14
Ensembl:   ENSG00000182256
Entrez:   2567
Chromosome Mapping:   15q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.590000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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