PrimePCR™ Probe Assay: ITGA3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057746
Assay Design:   exonic
Chromosome Location:   17:48156197-48156442question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000320031 ENST00000007722 ENST00000506827

Gene Information

The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   ITGA3
Gene Name:   integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
Aliases:   CD49C, FLJ34631, FLJ34704, GAP-B3, GAPB3, MSK18, VCA-2, VL3A, VLA3a
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000005884
Entrez:   3675
UniGene:   Hs.265829
Chromosome Mapping:   17q21.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.950000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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