PrimePCR™ Probe Assay: TRA2B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057696
Assay Design:   exonic
Chromosome Location:   3:185639899-185641673question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000453386 ENST00000259043 ENST00000382191

Gene Information

This gene encodes a nuclear protein which functions as sequence-specific serine/arginine splicing factor which plays a role in mRNA processing splicing patterns and gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq Aug 2011]

Gene Symbol:   TRA2B
Gene Name:   transformer 2 beta homolog (Drosophila)
Aliases:   DKFZp686F18120, Htra2-beta, SFRS10, SRFS10, TRA2-BETA, TRAN2B
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000136527
Entrez:   6434
UniGene:   Hs.533122
Chromosome Mapping:   3q26.2-q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.590000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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