PrimePCR™ Probe Assay: SPHK1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: SPHK1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPHK1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057678
Assay Design:   exonic
Chromosome Location:   17:74383045-74383194question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000545180 ENST00000592299 ENST00000590959 ENST00000323374 ENST00000392496

Gene Information

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P) a lipid mediator with both intra- and extracellular functions. Intracellularly S1P regulates proliferation and survival and extracellularly it is a ligand for cell surface G protein-coupled receptors. This protein and its product S1P play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory antiapoptotic and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2011]

Gene Symbol:   SPHK1
Gene Name:   sphingosine kinase 1
Aliases:   SPHK
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000176170
Entrez:   8877
Chromosome Mapping:   17q25.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.230000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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