PrimePCR™ Probe Assay: TOP3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057675
Assay Design:   exonic
Chromosome Location:   22:22326985-22328747question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000357179 ENST00000398793 ENST00000424393 ENST00000437929 ENST00000430142 ENST00000413067

Gene Information

This gene encodes a DNA topoisomerase an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however not all variants have been fully described. [provided by RefSeq Jul 2008]

Gene Symbol:   TOP3B
Gene Name:   topoisomerase (DNA) III beta
Aliases:   FLJ39376
RefSeq:   NC_000022.10 NT_011520.12
Ensembl:   ENSG00000100038
Entrez:   8940
UniGene:   Hs.436401
Chromosome Mapping:   22q11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.530000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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