PrimePCR™ Probe Assay: TSC22D3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057574
Assay Design:   exonic
Chromosome Location:   X:106957218-106957347question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000372390 ENST00000372397 ENST00000315660 ENST00000372383 ENST00000372384 ENST00000372382

Gene Information

The protein encoded by this gene shares significant sequence identity with the murine TSC-22 and Drosophila shs both of which are leucine zipper proteins that function as transcriptional regulators. The expression of this gene is stimulated by glucocorticoids and interleukin 10 and it appears to play a key role in the anti-inflammatory and immunosuppressive effects of this steroid and chemokine. Transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   TSC22D3
Gene Name:   TSC22 domain family, member 3
Aliases:   DIP, DKFZp313A1123, DSIPI, GILZ, TSC-22R, hDIP
RefSeq:   NC_000023.10 NT_011651.17
Ensembl:   ENSG00000157514
Entrez:   1831
UniGene:   Hs.731518
Chromosome Mapping:   Xq22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.320000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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