PrimePCR™ Probe Assay: SLC6A8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC6A8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC6A8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057531
Assay Design:   exonic
Chromosome Location:   X:152959389-152959603question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000313368 ENST00000373563 ENST00000409480 ENST00000409547 ENST00000423659 ENST00000409196 ENST00000409451 ENST00000440945 ENST00000373566 ENST00000452341 ENST00000326972 ENST00000424053 ENST00000429201 ENST00000194130 ENST00000539873 ENST00000296490 ENST00000400274 ENST00000417564 ENST00000318711 ENST00000427143 ENST00000409718 ENST00000358677 ENST00000409988 ENST00000409385 ENST00000360760 ENST00000409140 ENST00000253122 ENST00000430077 ENST00000442457 ENST00000457723 ENST00000369864 ENST

Gene Information

The protein encoded by this gene is a plasma membrane protein whose function is to transport creatine into and out of cells. Defects in this gene can result in X-linked creatine deficiency syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2008]

Gene Symbol:   SLC6A8
Gene Name:   solute carrier family 6 (neurotransmitter transporter, creatine), member 8
Aliases:   CRT, CRTR, CT1, MGC87396
RefSeq:   NC_000023.10 NT_167198.1 NG_012016.1
Ensembl:   ENSG00000130821
Entrez:   6535
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.160000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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