PrimePCR™ Probe Assay: SNX8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: SNX8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SNX8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057523
Assay Design:   exonic
Chromosome Location:   7:2309274-2311595question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000568225 ENST00000415766 ENST00000561639 ENST00000388833 ENST00000360658 ENST00000597542 ENST00000494383 ENST00000380903 ENST00000545124 ENST00000405546 ENST00000404914 ENST00000323019 ENST00000395704 ENST00000395706 ENST00000222990 ENST00000435060 ENST00000457286 ENST00000435336 ENST00000375254 ENST00000375267 ENST00000375217 ENST00000372814 ENST00000372807 ENST00000372791 ENST00000393533 ENST00000546203 ENST00000372796 ENST00000351030 ENST00000303890 ENST00000448249 ENST00000279394 ENST

Gene Information

Description Not Available

Gene Symbol:   SNX8
Gene Name:   sorting nexin 8
Aliases:   DKFZp761E1721, Mvp1
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000106266
Entrez:   29886
Chromosome Mapping:   7p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.780000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download