PrimePCR™ Probe Assay: PTCD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057522
Assay Design:   exonic
Chromosome Location:   7:99032428-99032534question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000292478 ENST00000430982 ENST00000430029 ENST00000555673 ENST00000413834 ENST00000280772 ENST00000373827 ENST00000373820 ENST00000355288 ENST00000503366

Gene Information

Description Not Available

Gene Symbol:   PTCD1
Gene Name:   pentatricopeptide repeat domain 1
Aliases:   KIAA0632
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000106246
Entrez:   26024
UniGene:   Hs.632313
Chromosome Mapping:   7q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.940000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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