PrimePCR™ Probe Assay: BCAR1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057512
Assay Design:   exonic
Chromosome Location:   16:75276810-75276946question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000345512 ENST00000373779 ENST00000428026 ENST00000460170 ENST00000356870 ENST00000323874 ENST00000162330 ENST00000393422 ENST00000535626 ENST00000538440 ENST00000418647 ENST00000542031 ENST00000420641 ENST00000393420 ENST00000568864 ENST00000564028 ENST00000566465 ENST00000569006 ENST00000561970 ENST00000563323 ENST00000567215 ENST00000546196

Gene Information

BCAR1 or CAS is an Src (MIM 190090) family kinase substrate involved in various cellular events including migration survival transformation and invasion (Sawada et al. 2006 [PubMed 17129785]).[supplied by OMIM May 2009]

Gene Symbol:   BCAR1
Gene Name:   breast cancer anti-estrogen resistance 1
Aliases:   CAS, CAS1, CASS1, CRKAS, FLJ12176, FLJ45059, P130Cas
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000050820
Entrez:   9564
UniGene:   Hs.479747
Chromosome Mapping:   16q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.650000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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