PrimePCR™ Probe Assay: SLC25A39, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0057509
Assay Design:   exonic
Chromosome Location:   17:42398854-42399094question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000225308 ENST00000377095 ENST00000590194 ENST00000586016 ENST00000537904 ENST00000588767 ENST00000588049 ENST00000585636 ENST00000373218

Gene Information

SLC25A39 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al. 2006 [PubMed 16949250]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC25A39
Gene Name:   solute carrier family 25, member 39
Aliases:   CGI69, FLJ22407
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000013306
Entrez:   51629
UniGene:   Hs.514216
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.990000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download