PrimePCR™ Probe Assay: HP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HP, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0056187
Assay Design:   exonic
Chromosome Location:   16:72088549-72090434question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000419835 ENST00000229854 ENST00000596288 ENST00000261588 ENST00000356166 ENST00000287468 ENST00000568722 ENST00000570083 ENST00000355906 ENST00000569639 ENST00000564499 ENST00000357763 ENST00000562526 ENST00000565574 ENST00000568417 ENST00000576168 ENST00000567185 ENST00000567612 ENST00000398131 ENST00000540303 ENST00000561690 ENST00000356967 ENST00000228226 ENST00000505231 ENST00000435205 ENST00000307808 ENST00000395146 ENST00000329759

Gene Information

This gene encodes a preproprotein which is processed to yield both alpha and beta chains which subsequently combine as a tetramer to produce haptoglobin. Haptoglobin functions to bind free plasma hemoglobin which allows degradative enzymes to gain access to the hemoglobin while at the same time preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin. Mutations in this gene and/or its regulatory regions cause ahaptoglobinemia or hypohaptoglobinemia. This gene has also been linked to diabetic nephropathy the incidence of coronary artery disease in type 1 diabetes Crohn's disease inflammatory disease behavior primary sclerosing cholangitis susceptibility to idiopathic Parkinson's disease and a reduced incidence of Plasmodium falciparum malaria. A similar duplicated gene is located next to this gene on chromosome 16. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   HP
Gene Name:   haptoglobin
Aliases:   BP, HP2ALPHA2, HPA1S, MGC111141
RefSeq:   NC_000016.9 NT_010498.15 NG_012651.1
Ensembl:   ENSG00000257017
Entrez:   3240
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.450000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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