PrimePCR™ Probe Assay: KRTAP2-3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055824
Assay Design:   exonic
Chromosome Location:   17:39215606-39215751question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000570303 ENST00000398556 ENST00000398547 ENST00000373658 ENST00000373655 ENST00000398542 ENST00000527361 ENST00000398538 ENST00000257070 ENST00000440175 ENST00000391418

Gene Information

Description Not Available

Gene Symbol:   KRTAP2-3
Gene Name:   keratin associated protein 2-3
Aliases:   KAP2.3, KRTAP2.3
RefSeq:   Not Available
Ensembl:   ENSG00000212724
Entrez:   85295
UniGene:   Not Available
Chromosome Mapping:   17q12-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.991600
y-intercept 36.770000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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