PrimePCR™ Probe Assay: KRTAP5-1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $208.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055799
Assay Design:   exonic
Chromosome Location:   11:1605595-1605696question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000382171 ENST00000318217 ENST00000394649 ENST00000350777 ENST00000527410 ENST00000536525 ENST00000397909 ENST00000228327 ENST00000266692 ENST00000415826

Gene Information

Description Not Available

Gene Symbol:   KRTAP5-1
Gene Name:   keratin associated protein 5-1
Aliases:   KRN1L, KRTAP5.1
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000205869
Entrez:   387264
UniGene:   Hs.632147
Chromosome Mapping:   11p15.5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.400000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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