PrimePCR™ Probe Assay: CPT1B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: CPT1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: CPT1B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055780
Assay Design:   exonic
Chromosome Location:   22:51015891-51016291question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000378108 ENST00000301332 ENST00000398898 ENST00000417901 ENST00000453664 ENST00000398892 ENST00000434032 ENST00000306026 ENST00000321110 ENST00000265593 ENST00000344937 ENST00000434054 ENST00000457512 ENST00000423355 ENST00000371712 ENST00000375231 ENST00000223428 ENST00000405237 ENST00000312108 ENST00000395650 ENST00000417176 ENST00000360719 ENST00000457250 ENST00000440709 ENST00000434492 ENST00000340446

Gene Information

The protein encoded by this gene a member of the carnitine/choline acetyltransferase family is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq Jun 2009]

Gene Symbol:   CPT1B
Gene Name:   carnitine palmitoyltransferase 1B (muscle)
Aliases:   CPT1-M, CPT1M, CPTI, CPTI-M, FLJ55729, FLJ58750, KIAA1670, M-CPT1, MCCPT1, MCPT1
RefSeq:   NC_000022.10 NT_011526.7 NG_012643.1
Ensembl:   ENSG00000205560
Entrez:   1375
Chromosome Mapping:   22q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.340000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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