PrimePCR™ Probe Assay: LRBA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: LRBA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LRBA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055595
Assay Design:   exonic
Chromosome Location:   4:151765814-151769974question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000510413 ENST00000357115 ENST00000509835 ENST00000507224 ENST00000502839 ENST00000535741

Gene Information

The protein encoded by this gene is a member of the WDL-BEACH-WD (WBW) gene family. Its expression is induced in B cells and macrophages by bacterial lipopolysaccharides (LPS). The encoded protein associates with protein kinase A and may be involved in leading intracellular vesicles to activated receptor complexes which aids in the secretion and/or membrane deposition of immune effector molecules. Defects in this gene are a cause of Chediak-Higashi syndrome (CHS1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]

Gene Symbol:   LRBA
Gene Name:   LPS-responsive vesicle trafficking, beach and anchor containing
Aliases:   BGL, CDC4L, DKFZp686A09128, DKFZp686K03100, DKFZp686P2258, FLJ16600, FLJ25686, LAB300, LBA, MGC72098
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000198589
Entrez:   987
Chromosome Mapping:   4q31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 36.720000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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