PrimePCR™ Probe Assay: ZNF28, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF28, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF28, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055583
Assay Design:   exonic
Chromosome Location:   19:53304795-53304929question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000438150 ENST00000457749 ENST00000391783 ENST00000596559 ENST00000594602 ENST00000339844 ENST00000360272 ENST00000414252 ENST00000595646 ENST00000243639 ENST00000597924 ENST00000396409 ENST00000390651 ENST00000442668 ENST00000545558 ENST00000358855

Gene Information

Description Not Available

Gene Symbol:   ZNF28
Gene Name:   zinc finger protein 28
Aliases:   DKFZp781D0275, KOX24
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000198538
Entrez:   7576
Chromosome Mapping:   19q

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994300
y-intercept 38.110000
Efficiency 87

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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