PrimePCR™ Probe Assay: GPAA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: GPAA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: GPAA1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055497
Assay Design:   exonic
Chromosome Location:   8:145139070-145139195question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000564504 ENST00000565405 ENST00000562454 ENST00000567546 ENST00000355091 ENST00000525087 ENST00000361036 ENST00000524418 ENST00000329875 ENST00000403172 ENST00000577756 ENST00000584848 ENST00000402252 ENST00000570156 ENST00000359599

Gene Information

Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence 1 cAMP- and cGMP-dependent protein kinase phosphorylation site 1 leucine zipper pattern 2 potential N-glycosylation sites and 8 putative transmembrane domains. [provided by RefSeq Jul 2008]

Gene Symbol:   GPAA1
Gene Name:   glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast)
Aliases:   GAA1, hGAA1
RefSeq:   NC_000008.10 NT_008046.16 NW_003315923.1
Ensembl:   ENSG00000197858
Entrez:   8733
Chromosome Mapping:   8q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.720000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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