PrimePCR™ Probe Assay: SLC28A3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC28A3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC28A3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055463
Assay Design:   exonic
Chromosome Location:   9:86893041-86893189question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000376238 ENST00000537648

Gene Information

Nucleoside transporters such as SLC28A3 regulate multiple cellular processes including neurotransmission vascular tone adenosine concentration in the vicinity of cell surface receptors and transport and metabolism of nucleoside drugs. SLC28A3 shows broad specificity for pyrimidine and purine nucleosides (Ritzel et al. 2001 [PubMed 11032837]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC28A3
Gene Name:   solute carrier family 28 (sodium-coupled nucleoside transporter), member 3
Aliases:   CNT3
RefSeq:   NC_000009.11 NT_008470.19
Ensembl:   ENSG00000197506
Entrez:   64078
Chromosome Mapping:   9q22.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.380000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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