PrimePCR™ Probe Assay: PCBP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PCBP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PCBP2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055414
Assay Design:   exonic
Chromosome Location:   12:53853181-53854897question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000333039 ENST00000534313 ENST00000394224 ENST00000377722 ENST00000246190 ENST00000375238 ENST00000393409 ENST00000251772 ENST00000262304 ENST00000423118 ENST00000374408 ENST00000408973 ENST00000508471 ENST00000418004 ENST00000451435 ENST00000435154 ENST00000449291 ENST00000340490 ENST00000426292 ENST00000276844 ENST00000436535 ENST00000298694 ENST00000298693 ENST00000371929 ENST00000355699 ENST00000356589 ENST00000402775 ENST00000307486 ENST00000454744 ENST00000334770 ENST00000588430 ENST

Gene Information

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1 this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA human papillomavirus type 16 L2 mRNA and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1 an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PCBP2
Gene Name:   poly(rC) binding protein 2
Aliases:   HNRPE2, MGC110998, hnRNP-E2
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000197111
Entrez:   5094
Chromosome Mapping:   12q13.12-q13.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.430000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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