PrimePCR™ Probe Assay: ZNF431, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ZNF431, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF431, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055377
Assay Design:   exonic
Chromosome Location:   19:21350386-21365434question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000261560 ENST00000599548 ENST00000594110 ENST00000595401 ENST00000596053 ENST00000596143 ENST00000596367 ENST00000601416 ENST00000291770 ENST00000600692 ENST00000599296 ENST00000311048 ENST00000598331 ENST00000358491 ENST00000597078 ENST00000397121 ENST00000357774 ENST00000593657 ENST00000601553 ENST00000456783 ENST00000594710 ENST00000418100 ENST00000597537 ENST00000597037 ENST00000599743 ENST00000300619 ENST00000601935 ENST00000600313 ENST00000596211 ENST00000359788 ENST00000599535 ENST

Gene Information

Description Not Available

Gene Symbol:   ZNF431
Gene Name:   zinc finger protein 431
Aliases:   DKFZp313E0830, KIAA1969
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000196705
Entrez:   170959
Chromosome Mapping:   19p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.220000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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