PrimePCR™ Probe Assay: LAMA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: LAMA2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LAMA2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055361
Assay Design:   exonic
Chromosome Location:   6:129641691-129649445question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000421865

Gene Information

Laminin an extracellular protein is a major component of the basement membrane. It is thought to mediate the attachment migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. It is composed of three subunits alpha beta and gamma which are bound to each other by disulfide bonds into a cross-shaped molecule. This gene encodes the alpha 2 chain which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Mutations in this gene have been identified as the cause of congenital merosin-deficient muscular dystrophy. Two transcript variants encoding different proteins have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LAMA2
Gene Name:   laminin, alpha 2
Aliases:   LAMM
RefSeq:   NC_000006.11 NG_008678.1 NT_025741.15
Ensembl:   ENSG00000196569
Entrez:   3908
Chromosome Mapping:   6q22-q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.240000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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