PrimePCR™ Probe Assay: ZNF75D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: ZNF75D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ZNF75D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0055087
Assay Design:   exonic
Chromosome Location:   X:134420045-134420181question
Amplicon Length:   107
Splice Variants Targeted:   ENST00000370766 ENST00000370764 ENST00000320955 ENST00000226432 ENST00000513409 ENST00000593516 ENST00000597484

Gene Information

This gene encodes a protein that likely functions as a transcription factor. The protein which belongs to the ZNF75 family includes an N-terminal SCAN domain a KRAB box and five C2H2-type zinc finger motifs. Another functional gene belonging to this family is located on chromosome 16 while pseudogenes have been identified on chromosomes 11 and 12. Alternative splicing results in multiple transcripts variants. [provided by RefSeq Jun 2010]

Gene Symbol:   ZNF75D
Gene Name:   zinc finger protein 75D
Aliases:   D8C6, MGC126327, ZNF75, ZNF82
RefSeq:   NC_000023.10 NT_011786.16 NG_021412.1
Ensembl:   ENSG00000186376
Entrez:   7626
Chromosome Mapping:   Xq26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.640000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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