PrimePCR™ Probe Assay: RAB11B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RAB11B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAB11B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054990
Assay Design:   exonic
Chromosome Location:   19:8464793-8464918question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000300527 ENST00000436769 ENST00000409416 ENST00000397763 ENST00000357838 ENST00000310645 ENST00000600719 ENST00000328024 ENST00000594216

Gene Information

The Ras superfamily of small GTP-binding proteins which includes the Ras (see MIM 190020) Ral (see MIM 179550) Rho (see MIM 165390) Rap (see MIM 179520) and Rab (see MIM 179508) families is involved in controlling a diverse set of essential cellular functions. The Rab family including RAB11B appears to play a critical role in regulating exocytotic and endocytotic pathways (summary by Zhu et al. 1994 [PubMed 7811277]).[supplied by OMIM Nov 2010]

Gene Symbol:   RAB11B
Gene Name:   RAB11B, member RAS oncogene family
Aliases:   H-YPT3, MGC133246
RefSeq:   NC_000019.9 NT_077812.2
Ensembl:   ENSG00000185236
Entrez:   9230
Chromosome Mapping:   19p13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.280000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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