PrimePCR™ Probe Assay: LRRC26, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: LRRC26, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: LRRC26, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054948
Assay Design:   exonic
Chromosome Location:   9:140063466-140063583question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000371542 ENST00000316409 ENST00000397558 ENST00000465645 ENST00000570475 ENST00000257821 ENST00000359866 ENST00000382963 ENST00000422132 ENST00000361115 ENST00000256413 ENST00000382998 ENST00000295874 ENST00000474889 ENST00000343932 ENST00000174618 ENST00000502597 ENST00000256497 ENST00000297623 ENST00000447116 ENST00000429348 ENST00000367338 ENST00000362061 ENST00000300482 ENST00000397928 ENST00000397932 ENST00000300481 ENST00000429646 ENST00000367080 ENST00000303177 ENST00000519867 ENST

Gene Information

Description Not Available

Gene Symbol:   LRRC26
Gene Name:   leucine rich repeat containing 26
Aliases:   CAPC, bA350O14.10
RefSeq:   NC_000009.11 NG_011507.1 NT_024000.16
Ensembl:   ENSG00000184709
Entrez:   389816
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.150000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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