PrimePCR™ Probe Assay: PDE4B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PDE4B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PDE4B, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054941
Assay Design:   exonic
Chromosome Location:   1:66827357-66827470question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000340635 ENST00000360047 ENST00000507116 ENST00000358923 ENST00000317118 ENST00000503258 ENST00000405755 ENST00000502484 ENST00000505453 ENST00000546160 ENST00000329654 ENST00000341517 ENST00000423207 ENST00000528771 ENST00000371045 ENST00000531025 ENST00000526197 ENST00000480109 ENST00000371049

Gene Information

This gene is a member of the type IV cyclic AMP (cAMP)-specific cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals such as hormones light and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq Jul 2008]

Gene Symbol:   PDE4B
Gene Name:   phosphodiesterase 4B, cAMP-specific
Aliases:   DKFZp686F2182, DPDE4, MGC126529, PDE4B5, PDEIVB
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000184588
Entrez:   5142
Chromosome Mapping:   1p31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995700
y-intercept 34.990000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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