PrimePCR™ Probe Assay: NPB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: NPB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: NPB, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054900
Assay Design:   exonic
Chromosome Location:   17:79860559-79860661question
Amplicon Length:   73
Splice Variants Targeted:   ENST00000320631 ENST00000359393 ENST00000573081 ENST00000333383 ENST00000538936 ENST00000376817 ENST00000512805 ENST00000504726 ENST00000512968 ENST00000503081 ENST00000513027 ENST00000456394 ENST00000381317 ENST00000381333 ENST00000416733 ENST00000534940 ENST00000254351 ENST00000381150 ENST00000362003 ENST00000505655 ENST00000521023 ENST00000430267 ENST00000403625 ENST00000403666 ENST00000405643 ENST00000261569 ENST00000443808 ENST00000404260 ENST00000321725 ENST00000388942 ENST00000360428 ENST

Gene Information

Neuropeptide B (NPB) is an endogenous peptide ligand for G protein-coupled receptor-7 (GPR7; MIM 600730).[supplied by OMIM Apr 2004]

Gene Symbol:   NPB
Gene Name:   neuropeptide B
Aliases:   PPL7, PPNPB
RefSeq:   NC_000017.10 NT_010663.15
Ensembl:   ENSG00000183979
Entrez:   256933
Chromosome Mapping:   17q25.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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