PrimePCR™ Probe Assay: RGS6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RGS6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RGS6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054807
Assay Design:   exonic
Chromosome Location:   14:72985114-72985243question
Amplicon Length:   100
Splice Variants Targeted:   ENST00000553525 ENST00000555571 ENST00000553530 ENST00000556437 ENST00000355512 ENST00000404301 ENST00000406236 ENST00000407322 ENST00000434263 ENST00000554782 ENST00000402788 ENST00000343854

Gene Information

This gene encodes a member of the RGS (regulator of G protein signaling) family of proteins which are defined by the presence of a RGS domain that confers the GTPase-activating activity of these proteins toward certain G alpha subunits. This protein also belongs to a subfamily of RGS proteins characterized by the presence of DEP and GGL domains the latter a G beta 5-interacting domain. The RGS proteins negatively regulate G protein signaling and may modulate neuronal cardiovascular lymphocytic activities and cancer risk. Many alternatively spliced transcript variants encoding different isoforms with long or short N-terminal domains complete or incomplete GGL domains and distinct C-terminal domains have been described for this gene however the full-length nature of some of these variants is not known.[provided by RefSeq Mar 2011]

Gene Symbol:   RGS6
Gene Name:   regulator of G-protein signaling 6
Aliases:   DKFZp313G1241, FLJ43552, GAP, MGC142132
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000182732
Entrez:   9628
Chromosome Mapping:   14q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.960000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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