PrimePCR™ Probe Assay: BOK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: BOK, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BOK, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054462
Assay Design:   exonic
Chromosome Location:   2:242513019-242513119question
Amplicon Length:   71
Splice Variants Targeted:   ENST00000318407

Gene Information

The protein encoded by this gene belongs to the BCL2 family members of which form homo- or heterodimers and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues interacts strongly with some antiapoptotic BCL2 proteins not at all with proapoptotic BCL2 proteins and induces apoptosis in transfected cells. Thus this protein represents a proapoptotic member of the BCL2 family. [provided by RefSeq Sep 2011]

Gene Symbol:   BOK
Gene Name:   BCL2-related ovarian killer
Aliases:   BCL2L9, BOKL, MGC4631
RefSeq:   NC_000002.11 NT_005416.13
Ensembl:   ENSG00000176720
Entrez:   666
Chromosome Mapping:   2q37.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.430000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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