PrimePCR™ Probe Assay: PPP2R2D, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PPP2R2D, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PPP2R2D, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054370
Assay Design:   exonic
Chromosome Location:   10:133769342-133769486question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000394413 ENST00000508545 ENST00000394414 ENST00000394411 ENST00000336640 ENST00000504198 ENST00000394409 ENST00000356826 ENST00000453001 ENST00000394410 ENST00000335585 ENST00000506140 ENST00000515571 ENST00000382599 ENST00000507294 ENST00000455566 ENST00000422256 ENST00000595776 ENST00000597594

Gene Information

Description Not Available

Gene Symbol:   PPP2R2D
Gene Name:   protein phosphatase 2, regulatory subunit B, delta
Aliases:   MDS026
RefSeq:   NC_000010.10 NT_008818.16
Ensembl:   ENSG00000175470
Entrez:   55844
Chromosome Mapping:   10q26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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