PrimePCR™ Probe Assay: SLC25A30, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SLC25A30, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SLC25A30, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054289
Assay Design:   exonic
Chromosome Location:   13:45978537-45980100question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000519676 ENST00000519547 ENST00000522438 ENST00000539591

Gene Information

Although the outer mitochondrial membrane is permeable to many small metabolites transport of solutes across the inner mitochondrial membrane is achieved by members of the mitochondrial carrier protein family such as SLC25A30 (Haguenauer et al. 2005 [PubMed 15809292]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC25A30
Gene Name:   solute carrier family 25, member 30
Aliases:   KMCP1
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000174032
Entrez:   253512
Chromosome Mapping:   13q14.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.720000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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