PrimePCR™ Probe Assay: MRPL52, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MRPL52, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MRPL52, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054179
Assay Design:   exonic
Chromosome Location:   14:23303433-23303570question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000544264 ENST00000164024 ENST00000427997 ENST00000341068 ENST00000220809 ENST00000352041 ENST00000270189 ENST00000311620 ENST00000295844 ENST00000274605 ENST00000355151 ENST00000397496 ENST00000555345 ENST00000432849 ENST00000553711 ENST00000397505 ENST00000557221 ENST00000311892 ENST00000556840 ENST00000555536 ENST00000278823 ENST00000524902 ENST00000232854 ENST00000222990 ENST00000251089 ENST00000231683 ENST00000366609

Gene Information

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species the proteins comprising the mitoribosome differ greatly in sequence and sometimes in biochemical properties which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which has no bacterial homolog. Multiple transcript variants encoding different protein isoforms were identified through sequence analysis. [provided by RefSeq Jul 2008]

Gene Symbol:   MRPL52
Gene Name:   mitochondrial ribosomal protein L52
Aliases:   Not Available
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000172590
Entrez:   122704
Chromosome Mapping:   14q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 36.280000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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