PrimePCR™ Probe Assay: SPTLC3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SPTLC3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SPTLC3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054152
Assay Design:   exonic
Chromosome Location:   20:13029658-13052905question
Amplicon Length:   93
Splice Variants Targeted:   ENST00000434210 ENST00000399002 ENST00000450297 ENST00000378194 ENST00000398457 ENST00000347800 ENST00000398449 ENST00000361802 ENST00000343687 ENST00000398437 ENST00000340588 ENST00000428484 ENST00000354639 ENST00000433467 ENST00000355870

Gene Information

The SPTLC3 gene encodes an isoform of the third subunit of serine palmitoyltransferase (SPT; EC 2.3.1.50) which catalyzes the rate-limiting step of the de novo synthesis of sphingolipids (Hornemann et al. 2006 [PubMed 17023427]). SPT contains 2 main subunits: the common SPTLC1 subunit (MIM 605712) and either SPTLC2 (MIM 605713) or its isoform SPTLC2L (SPTLC3) depending on the tissue in which biosynthesis occurs (Hornemann et al. 2006 [PubMed 17023427]). There are also 2 highly related isoforms of a third subunit SSSPTA (MIM 613540) and SSSPTB (MIM 610412) that confer acyl-CoA preference of the SPT enzyme and are essential for maximal enzyme activity (Han et al. 2009 [PubMed 19416851]).[supplied by OMIM Nov 2010]

Gene Symbol:   SPTLC3
Gene Name:   serine palmitoyltransferase, long chain base subunit 3
Aliases:   C20orf38, FLJ11112, FLJ90790, LCB2B, LCB3, SPT3, SPTLC2L, dJ718P11, dJ718P11.1
RefSeq:   NC_000020.10 NT_011387.8
Ensembl:   ENSG00000172296
Entrez:   55304
Chromosome Mapping:   20p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.910000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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