PrimePCR™ Probe Assay: TLN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: TLN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: TLN2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0054115
Assay Design:   exonic
Chromosome Location:   15:63054515-63055764question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000561311 ENST00000494733 ENST00000472902 ENST00000306829

Gene Information

This gene encodes a protein related to talin 1 a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but like talin 1 is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq Jul 2008]

Gene Symbol:   TLN2
Gene Name:   talin 2
Aliases:   DKFZp451B1011, DKFZp686I0976, DKFZp686K0979, ILWEQ, KIAA0320
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000171914
Entrez:   83660
Chromosome Mapping:   15q15-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 38.340000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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