PrimePCR™ Probe Assay: VSTM2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053963
Assay Design:   exonic
Chromosome Location:   7:54614677-54617634question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000302287 ENST00000407838 ENST00000404951 ENST00000402613 ENST00000402026

Gene Information

Description Not Available

Gene Symbol:   VSTM2A
Gene Name:   V-set and transmembrane domain containing 2A
Aliases:   MGC33530, VSTM2
RefSeq:   NC_000007.13 NT_033968.6
Ensembl:   ENSG00000170419
Entrez:   222008
UniGene:   Hs.731937
Chromosome Mapping:   7p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.580000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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