PrimePCR™ Probe Assay: UBE2E3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: UBE2E3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: UBE2E3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053943
Assay Design:   exonic
Chromosome Location:   2:181846753-181846864question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000397081 ENST00000409036 ENST00000397087 ENST00000264111 ENST00000538974 ENST00000540783 ENST00000539331 ENST00000535187 ENST00000602303 ENST00000602959 ENST00000602479 ENST00000602291 ENST00000410062 ENST00000602475 ENST00000602499 ENST00000602710 ENST00000602632 ENST00000392415

Gene Information

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes or E1s ubiquitin-conjugating enzymes or E2s and ubiquitin-protein ligases or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts which indicates that this enzyme is highly conserved in eukaryotes. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   UBE2E3
Gene Name:   ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog, yeast)
Aliases:   UBCH9, UbcM2
RefSeq:   NC_000002.11 NT_005403.17
Ensembl:   ENSG00000170035
Entrez:   10477
Chromosome Mapping:   2q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.890000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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